Optimal Concentration of OSM for Wound Healing Activity of Fibroblasts

J Korean Wound Management Soc > Volume 2(2); 2006 > Article

Original

Journal of the Korean Wound Management Society 2006;2(2): 77-82.

섬유아세포의 창상치유 촉진을 위한 OSM의 적정농도

전경욱·한승규·이병일·김우경

고려대학교 의과대학 성형외과학교실

Optimal Concentration of OSM for Wound Healing Activity of Fibroblasts

Kyung-Wook Chun, Seung-Kyu Han, Byung-Il Lee, Woo-Kyung Kim

Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea

Published online: 30 November 2006.

ABSTRACT

Oncostatin M (OSM) is a multifunctional cytokine that belongs to the interleukin (IL)-6 family. Although there have been a number of studies that focused on the role and mechanism of OSM in various organs and tissues, there are few reports on its effect on wound healing. The purposes of this study are to evaluate the effect of OSM on proliferation and matrix synthesis of dermal fibroblasts. In particular, this study focused on cell proliferation, collagen and glycosaminoglycan (GAG) syntheses (which are the major components of the extracellular matrices). Excess skin that was obtained from patients who underwent skin grafts, was used for this study. From this material, fibroblasts were isolated and cultured. The cultured fibroblasts were treated with one of four concentrations of OSM. The OSM concentrations used were 0, 50, 100, and 200 ng/ml. After the OSM treatment, cell proliferation was determined by the MTT assay, collagen synthesis by the C1CP method, GAG levels by the Blyscan Dye method. Subsequently, the results of each group were compared. OSM treatment increased all the components tested in the study. In particular, cell proliferation, GAG synthesis demonstrated statistically significant increases (p＜0.05 in the Mann-Whitney U-test). The highest increase in all the components was obtained at a 100 ng/ml concentration of OSM. The results from the present study indicate that OSM stimulates proliferation and matrix synthesis of human normal dermal fibroblast and the optimal concentration for wound healing is 100 ng/ml. (J Korean Wound Care Soc 2006;2:77-82)

Key Words: Oncostatin M, Dermal fibroblasts