Comparison of Cell Viability of Human Fibroblast in PLGA and PCL/SF Scaffold

J Korean Wound Management Soc > Volume 4(1); 2008 > Article

Original

Journal of the Korean Wound Management Society 2008;4(1): 6-11.

PLGA와 PCL/SF 스케폴드에서 인체섬유모세포의 세포친화성 비교

이진희ㆍ이상우ㆍ장학ㆍ민경원

서울대학교 의과대학 성형외과학교실, 성형재건연구소

Comparison of Cell Viability of Human Fibroblast in PLGA and PCL/SF Scaffold

Jin Hee Lee, Sang Woo Lee, Hak Chang, Kyung Won Minn

Department of Plastic & Reconstructive Surgery, Research Institute of Plastic & Reconstructive Surgery, College of Medicine, Seoul National University, Seoul, Korea

Published online: 31 May 2008.

ABSTRACT

The engineered scaffold substitute plays an important role in modulating cell and tissue growth. A novel poly (D,L-lactide-co-glycolide) (PLGA) and a Poly(Յ-caprolactone)/silk fibroin (PCL/SF) scaffold nanofiber structure with a unique architecture were produced by an electrospinning process for tissue-engineering applications. The electrospun structure, composed of PLGA and PCL/SF nanofibers, features a morphologic similarity to the extracellular matrix (ECM) of natural tissue, which is characterized by a wide range of pore diameter distribution, high porosity, and effective mechanical properties. These favorable cell-matrix structures within the cellular construct support the active biocompatibility of the structure. So, the electrospun nanofibrous structure is capable of supporting cell attachment and proliferation. Cells seeded on these structures tend to maintain phenotypic shape and viability according to nanofiber orientation. This study evaluates the comparison of the cellular morphology and viability of human fibroblasts following their seeding into PLGA and PCL/SF scaffolds. The cell viability estimated by MTT assay and microscopic examination. Our results appeared higher cell viability on the fibroblast seeded PCL/SF scaffold than PLGA scaffold. (J Korean Wound Management Soc 2008;4:6-11)

Key Words: Antimicrobial property poly(D,L-lactide-co-glycolide) (PLGA), Poly(Յ; -caprolactone)/silk fibroin (PCL/SF) scaffold, Human fibroblast, Cell viability