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J Korean Wound Management Soc > Volume 6(2); 2010 > Article
Journal of the Korean Wound Management Society 2010;6(2): 069-076.
햄스터의 심재성 2도 화상치유에 미치는 rrhGM-CSF의 효과
1순천향대학교 생명과학과, 2(주)차바이오앤디오스텍
Effect of rrhGM-CSF on Wound Healing in Hamster Model of Burn Trauma
Si Hyun Heo1, Ji Hyun Kim1, Kwang Ho Yoon1, Young Jun Lee2, Kyu Boem Han2, Wan Jong Kim1
1Department of Life Science, Soonchunhyang University, Asan, 2CHA Bio & Diostech Co., Ltd., Seoul, Korea
  Published online: 30 November 2010.
A burn is one of the most common and devastating forms of trauma. A number of approaches were taken in burn repair. GM-CSF is a multipotent growth factor, which also plays an important role during the process of wound healing. rrhGM-CSF, which contains more oligosaccharide side chains than any other types of GM-CSF, was specifically produced from rice cell culture in our laboratory (CHA Bio & Diostech Co., Ltd.). The present study was taken to evaluate the influence on burn (2nd degree) wound healing of rrhGM-CSF in male golden hamsters (Mesocricetus auratus). Methodologically, deep second-degree burn defects of 1.5 cm in diameter were made using a hot iron in the back skin of hamsters, and 100 Ռl base cream containing rrhGM-CSF 50 Ռg/ml (experimental groups) was applied. Control groups were given only base cream without rrhGM-CSF. The wound sizes of experimental groups were macroscopically reduced from 6 days after burn compared to those of control group and almost repaired by 15 days. Light microscopically, reepithelialization and architecture of the skin following injury were well accomplished in the experimental group. Immunohistochemically, positive reaction of PCNA which indicates cell proliferation in the skin following injury was more prominent in the experimental group. Since this type of GM-CSF is highly glycosylated, the effectiveness might be retain longer and stable during wound healing in the animal model. The results suggest that rrhGM-CSF can be used for further development of the therapeutic manipulation of burn wound healing. (J Korean Wound Management Soc 2010;6:69-76)
Key Words: Deep second-degree burn, rrhGM-CSF, Burn wound healing, Hamster
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