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Journal of the Korean Wound Management Society 2006;2(2): 64-67. |
| 골수기질세포의 증식능 및 세포외기질 합성능 |
| 임형우·한승규·윤태환·이병일·김우경 |
| 고려대학교 의과대학 성형외과학교실 |
| Proliferation and Extracellular Matrix Synthesis of Bone Marrow Stromal Cells |
| Hyung-Woo Lim, Seung-Kyu Han, Tae-Hwan Yoon, Byung-Il Lee, Woo-Kyung Kim |
| Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea |
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Published online: 30 November 2006. |
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| ABSTRACT |
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There has been no research on the effect of a graft of bone marrow stromal cells on wound healing. The wound healing porcess requires cell proliferation and production of extracellular matrix and various growth factors. The purpose of this study was to compare the abilities of human fibroblasts and bone marrow stromal cells, which contains mesenchymal stem cells, to proliferate and to produce collagen. Human bone marrow stromal cells and fibroblasts were isolated from bone marrow and dermis of the same patients and grown in culture respectively. Cell proliferation and production of type I collagen by human bone marrow stromal cells and dermal fribroblasts were examined by MTT method and by ELISA of cell culture media on day 1, 3, and 5 days post-incubating. The human bone marrow stromal cells showed 11∼17% higher cell proliferation than firboblasts at each time interval. The levels of type I collagen in the human bone marrow stromal cell group was also significantly higher than those in the fibroblast group. The results indicate that the grafts of human bone marrow stromal cells can show more promising effect than that of fibroblasts for ealing of chronic wounds. (J Korean Wound Care Soc 2006;2:64-67) |
| Key Words:
Stem cells, Collagen, Cell proliferation |
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