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J Korean Wound Management Soc > Volume 2(1); 2006 > Article
Journal of the Korean Wound Management Society 2006;2(1): 14-18.
피부섬유아세포의 단층배양에 있어 자가·동종·이종혈청 사용의 비교
이민아·한승규·김정배1·김우경
고려대학교 의과대학 성형외과학교실, 1건양대학교 의과대학 성형외과학교실
Comparison of Effect of Autogenic, Allogenic, Xenogenic Serum Supplementations in Fibroblast Culture
Min-Ah Lee, Seung-Kyu Han, Jeong-Bae Kim1, Woo-Kyung Kim
Department of Plastic Surgery, Korea University College of Medicine, Seoul, 1Department of Plastic Surgery, Konyang University College of Medicine, Daejeon, Korea
  Published online: 31 May 2005.
ABSTRACT
Expanding cells ex-vivo is important in tissue-engineering. Culture medium is usually supplemented with fetal bovine serum (FBS) in most of experiments. However, cells grown in bovine serum media have possibilities of disseminating bovine diseases and/or causing patient's immune reaction. To overcome these problems, autologous or homologous serum should be used instead of FBS. The purpose of this study is to compare cell proliferation and collagen synthesis depending on the kind of sera mixed on media and to provide the guideline on applying established experimental data to clinical cases. Human dermal fibroblasts were obtained from four patients. Five thousand cells per well in 96-well plates were incubated DMEM/F-12 Nutrient with varying serum mixture; 10% autologous serum, 10% homologous serum, and 10% FBS. Five days after incubation fibroblast proliferation and collagen production were determined by MTT assay and CICP enzyme immunoassay. The means of cell number were 3.95⁓104/well, 2.97⁓104/well, and 2.30⁓104/well, respectively. The average amounts of collagen synthesized were 238.13⁑15.6 ng/ml, 204.88⁑13.5 ng/ml, 163.88⁑9.9 ng/ml in each. These results show that use of human serum mixture may contribute to not only exclude infection of bovine diseases and/or the patient's immune reaction, but also increase cell proliferation and collagen synthesis. (J Korean Wound Care Soc 2006;2:14-18)
Key Words: Serum, Culture medium, Fibroblast, Collagen
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